The complete structural elucidation of GPI anchors is a complex process that requires relatively large amounts of starting Fresh frozen tissue samples from eight primary RCC lesions and autologou... Spinal muscular atrophy (SMA) is a neuromuscular disease resulting from mutations in the survival motor neuron 1 (SMN1) gene. were fixed and immunostained with an anti-NP monoclonal antibody. We recently discovered that the strongest genetic risk factor for sporadic AD, apolipoprotein E epsilon 4 ( APOE 4), exacerbates synapse loss and synaptic accumulation of oligomeric amyloid... AMPK acts downstream of the tumor suppressor LKB1, yet its role in cancer has been controversial. (PDF), All proteins with altered expression levels >20% in WldS striatal synaptosome preparations at 24 and/or 48 hrs after cortical lesion. Douglas Lamont नाम के लोगों की प्रोफ़ाइल देखें. Recognition and repair of cellular damage is crucial if organisms are to survive harmful environmental conditions. Douglas LAMONT, Proteomics Facility Manager | Cited by 2,575 | of University of Dundee, Dundee | Read 104 publications | Contact Douglas LAMONT Annie Keir Lamont … Document S1. In order to establish whether changes in CDK5 activity are associated w... Equine grass sickness (EGS) is an acute, predominantly fatal, multiple system neuropathy of grazing horses with reported incidence rates of approximately 2%. (PDF), All proteins with altered expression levels >20% in wild-type striatal synaptosome preparations at 24 and/or 48 hrs after cortical lesion. To determine whether HTS could be employed to detect the presence of DVGs in persistently infected cells, with or without the need for prior nucleocapsid purification, A549 cells were infected... Trypanosoma cruzi, the etiological agent of Chagas' disease, affects 8 million people predominantly living in socioeconomic underdeveloped areas. The autosomal recessive neurodegenerative disease spinal muscular atrophy (SMA) results from low levels of survival motor neuron (SMN) protein; however, it is unclear how reduced SMN promotes SMA development. As well as causing acute infections that result in mild to serious disease, many RNA viruses can establish prolonged or persistent infections in some infected individuals, that occasionally lead to chronic or reactive disease. © 2008-2021 ResearchGate GmbH. Many diabetic patients suffer from declining renal function without developing albuminuria. Total cel... Mass spectroscopy was used to map the phosphorylation sites on P of rPIV5-W3:P(S157) and rPIV5-W3:P(F157). The majority of A549 cells do not die and become persistently infected following high moi infections with PIV5-W3. Douglas Lamont Proteomics Facility Manager. 35F Douglas Road, Broughty Ferry, Dundee, DD4 7SN. Using mouse models of SMA, we observed widespr... Background: University students and faculty, institute members, and independent researchers, Technology or product developers, R&D specialists, and government or NGO employees in scientific roles, Health care professionals, including clinical researchers, Journalists, citizen scientists, or anyone interested in reading and discovering research. School of Life Sciences, University of Dundee, Dundee. As current pre-clinical models of NAFLD are invariably imperfect and generally take a long time to d... Cordycepin (3'-deoxyadenosine) is a major bioactive agent in Cordyceps militaris, a fungus used in traditional Chinese medicine. To identify alternative biomarkers for diabetic nephropathy (DN) we performed urinary peptidomic analysis in a rodent model in which hyperglycemia and hypertension synergize to promote renal pathologic changes consistent with human DN. Douglas and Angus (commonly referred to as Douglas) is an area of Eastern Dundee, Scotland.It is located between Whitfield to the North and Broughty Ferry to the East. The siRNA knockdown of IFN Regulatory Factor 5 (IRF5) in the human plasmacytoid dendritic cell line Gen2.2 prevented IFNβ production induced by compound CL097, a ligand for Toll-like receptor 7 (TLR7). Centre for Advanced Scientific Technologies, School of Life Sciences. The Scrymgeour family seat is still at Birkhill north of Cupar in Fife. Monolayers of A549 cells were either mock infected or infected with PIV5-W3 at 10 pfu/cell and at 24 and 96 h p.i. SMA patients are more susceptible to dyslipidemia as well as liver steatosis, features reproduced in SMA mouse models. Our clients include Universities, Research Institutes and companies in the UK, Europe, USA, South America and Asia. Known Locations: Salem VA 24153, Roanoke VA 24008, Roanoke VA 24014 Possible Relatives: John Henry Douglas, Lakeia Renee Douglas, Melissa June Douglas. However, little is known about the proteins and pathways regulated by S-glutathionylation in whole organisms and current approaches lack the sensitivity to examine this modification under basal conditions. Monolayers of A549 cells were either mock infected or infected with PIV5-W3 at 10 pfu/cell and at the times indicated the cells were metabolically labelled for 1h with [35S]-L-methionine and the viral proteins immune-precipitated. Current pre-clinical models of NAFLD are invariably imperfect and generally take a long time to develop. in cells unable to produce IFN. Search. Sorry, you need to be a researcher to join ResearchGate. The incidence of oesophageal adenocarcinoma is increasing worldwide but survival remains poor. Dundee Although several stage-specific genes have been identified in Leishmania, the molecular mechanisms governing developmental gene regulation in this organism are still not well understood. Clan Seat. It has been proposed to have many beneficial metabolic effects by activating AMP-activated protein kinase (AMPK), but the mechanism of activation remained uncertain. organism Trypanosoma brucei, phosphorylation on trypanosomal protein tyrosine residues has been rep... Phototropins (phot1 and phot2) are blue-light receptor kinases controlling a range of responses that optimize the photosynthetic efficiency of plants. We present state-of-art label-free quantitative proteomics method to assess proteome of renal cell carcinoma (RCC) compared with noncancer renal tissues. Amino acid residue numbers are indicated at the right-hand side of the Figure and the serin... Panel a) Transcription of PIV5-CPI+ mRNA synthesis is not inhibited at late times p.i. The nematodes Trichinella spiralis and Trichinella pseudospiralis are both intracellular parasites of skeletal muscle cells and induce profound alterations in the host cell resulting in a re-alignment of muscle-specific gene expression. Location. Defective viral genomes (DVGs) cannot be detected in A549 cells persistently infected with PIV5-W3 but are present in cells persistently infected with CPI+. * Corresponding author: Angus I. Lamond Phone: +44 (0)1382 385473 Fax: +44 (0)1382 385695 Quantitative methods for measuring mRNA expression do not reliably predict corresponding protein levels and provide little or no information on other... Low levels of full-length survival motor neuron (SMN) protein cause the motor neuron disease, spinal muscular atrophy (SMA). However, the downstream mechanisms through which α-synuclein controls synaptic stability and degeneration are not fully understood. Here, we combined morphological techniques, supe... E3 ubiquitin ligases are key enzymes within the ubiquitin proteasome system which catalyze the ubiquitination of proteins, targeting them for proteasomal degradation. View the profiles of people named Douglas Vlamont. Proteomics Facility Manager (Proteomics/Mass Spectrometry), Manager of a Core Proteomics/Mass Spectrometry Facility, SMN Depleted Mice Offer a Robust and Rapid Onset Model of Nonalcoholic Fatty Liver Disease, Aldolase is a sensor for both low and high glucose, linking to AMPK and mTORC1, Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease, Pre-natal manifestation of systemic developmental abnormalities in spinal muscular atrophy, A mouse model for spinal muscular atrophy provides insights into non-alcoholic fatty liver disease pathogenesis, Mechanism of Activation of AMPK by Cordycepin, Comparative profiling of the synaptic proteome from Alzheimer’s disease patients with focus on the APOE genotype, Accepted Manuscript Altered mitochondrial bioenergetics are responsible for the delay in Wallerian degeneration observed in neonatal mice Altered mitochondrial bioenergetics are responsible for the delay in Wallerian degeneration observed in neonatal mice, Distinct proteomic profiles in monozygotic twins discordant for ischaemic stroke, Comparative profiling of the synaptic proteome from Alzheimer's disease patients with focus on the APOE genotype, Phenformin, But Not Metformin, Delays Development of T Cell Acute Lymphoblastic Leukemia/Lymphoma via Cell-Autonomous AMPK Activation, Regional Molecular Mapping of Primate Synapses during Normal Healthy Aging, The switch between acute and persistent paramyxovirus infection caused by single amino acid substitutions in the RNA polymerase P subunit, Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix, UBA1/GARS-dependent pathways drive sensory-motor connectivity defects in spinal muscular atrophy, Proteomic profiling of neuronal mitochondria reveals modulators of synaptic architecture, Document S2. Homepage. Email. Archives. Phone +44 (0)1382 388275. Deafferentation of motor neurons as a result of defective sensory-motor connectivity is a critical early event in the pathogenesis of spinal muscular atrophy, but the underlying molecular pathways remain unknown. Neoadjuvant chemotherapy may improve survival, but targeting treatment to patients who respond to chemotherapy could be improved by the availability of markers of response. The Discovery Centre Although some of the major risk factors for stoke, such as smoking and hypertension, are well-documented, the underlying genetic and detailed molecular mechanisms remain elusive. And they examine the work of the Scottish Government. The lipid raft concept proposes that membrane environments enriched in cholesterol and sphingolipids cluster certain proteins and form platforms to integrate cell signaling. material. Inducing macromolecular interactions with small molecules to activate cellular signaling is a challenging goal. Douglas Lamont, age 38, Salem, VA 24153 View Full Report. See the complete profile on LinkedIn and discover John Douglas’ connections and jobs at similar companies. At the times indicated the cells were metabolically labe... Inhibition of PLK1 by BI 2536 did not significantly affect the kinetics of PIV5-W3 protein synthesis inhibition. However many of its substrates have roles in cell growth and oncogenesis, raising the possibility that CDK5 modulation could have therapeutic benefit. Monolayers of A549 cells grown in 25cm flasks were infected with PIV5-CPI+ at 10 pfu/cell, RNA was extracted at 6, 12, 18, 24, and 48 p.i. Dow Street Although motor neurons undoubtedly contribute directly to SMA pathogenesis, the role of muscle is less clear. We recently observed that the strongest genetic risk factor for sporadic AD, apolipoprotein E epsilon 4 (APOE4), is associated with exacerbated synapse loss and synaptic accumulatio... Neurodegenerative and neuromuscular disorders can manifest throughout the lifespan of an individual, from infant to elderly individuals. £123456 They have also lived in Nesconset, NY and Newton Grove, NC. The catalytic subunit of type 1 protein phosphatase (PP1C) interacts with a large number of polypeptides in eukaryotic cells from yeast to man and these regulatory subunits can both modulate the activity of PP1C and target it to different subcellular locations. cells. University of Dundee Proteomics Facility Managerd.j.lamont@dundee.ac.uk, FingerPrints Proteomics Facility In this paper, we assess the degree of structural information that can be obta... A major challenge in cell biology is to identify the subcellular distribution of proteins within cells and to characterize how protein localization changes under different cell growth conditions and in response to stress and other external signals. d.j.lamont@dundee.ac.uk; Tel: 01382 388275 (Office) Tel: 01382 384797 (Lab) Structural elucidation of the key ternary ligase–PROTAC–target specie... α-synuclein plays a central role in Parkinson's disease, where it contributes to the vulnerability of synapses to degeneration. Other Michael Douglas films that you might recognise include include Wall Street (1987) and The American… E3 ligases are gaining importance as targets to small molecules, both for direct inhibition and to be hijacked to induce the degradation of non-native neo-substrates using bivalent co... SU6656, a Src kinase inhibitor, was reported to increase fat oxidation and reduce body weight in mice, with proposed mechanisms involving AMP-activated protein kinase (AMPK) activation via inhibition of phosphorylation of either LKB1 or AMPK by the Src kinase, Fyn. SMN-restoring therapies have recently emerged; however, pre-clinical and clinical studies revealed a limited therapeutic time-window and systemic aspects of the disease. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes),... Postnatal synapse elimination plays a critical role in sculpting and refining neural connectivity throughout the central and peripheral nervous systems, including the removal of supernumerary axonal inputs from neuromuscular junctions (NMJs). A mouse model of SMN depletion (Smn2B/- mice) was recently shown to develop signif... CLN1 disease is a fatal inherited neurodegenerative lysosomal storage disease of early childhood, caused by mutations in the CLN1 gene, which encodes the enzyme Palmitoyl protein thioesterase-1 (PPT-1). CL097 also stimulated the phosphorylation of IRF5 at Ser462 and stimulated the nuclear translocation of wild-type IRF5, but not the IRF5[Ser462Ala]... Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Dundee United slip further behind at the bottom of the Premiership as Hamilton miss a late penalty in a drab draw. Spinal muscular atrophy (SMA) is an inherited neuromuscular disorder leading to paralysis and death in children. Douglas Lamont Proteomics Facility Manager d.j.lamont@dundee.ac.uk FingerPrints Proteomics Facility The Discovery Centre School of Life Sciences University of Dundee Dow Street Dundee DD1 5EH Tel: 01382 388275 (Office) Tel: 01382 384797 (Lab) See also Join Facebook to connect with Douglas Lamont and others you may know. We recently found significant spinal pathology in Ppt1-deficient (Ppt1-/-) mice and human CLN1 disease that contributes to clinical outcome and pre... Spinal muscular atrophy (SMA) is a neuromuscular disease caused by mutations in survival motor neuron 1 (SMN1). DD1 5EH, Tel: 01382 388275 (Office) Douglas Lamont Head of the Fingerprints Proteomics Facility. However, whether this is mediated by cell-autonomous AMPK activation within tumor progenitor cells has been... Normal mammalian brain aging is characterized by the selective loss of discrete populations of dendritic spines and synapses, particularly affecting neuroanatomical regions such as the hippocampus. Records may include photos, original documents, family history, relatives, specific dates, locations and full names. Protein localization is usually determined either by microscopy or by using cell fractionation combin... A Trypanosoma brucei TbGPI12 null mutant that is unable to express cell surface procyclins and free glycosylphosphatidylinositols (GPI) revealed This raises a fundamental question of whether SMA has pre-symptomati... Background & aims The neuromuscular junction (NMJ) plays a fundamental role in transferring information from lower motor neuron to skeletal muscle to generate movement. Supplemental Experimental Procedures, Figures S1–S4, and Tables S1–S3. Little is known about the molecular mechanisms involved in the establishment of such infections. We find that SteC promotes actin cytoskeleton reorganization by activating a signaling pathway involving the MAP kinases MEK and ERK, myosin li... Proteins with altered expression levels >20% in striatal synapse-enriched preparations from wild-type mice 48 hrs after cortical lesion (emPAI = exponentially modified protein abundance index). The protozoan parasite Trypanosoma brucei is the causative agent of human African sleeping sickness and related animal diseases, and it has over 170 predicted protein kinases. Supplemental Experimental Procedures, Figures S1–S5, and Tabled S1–S3, Combining Comparative Proteomics and Molecular Genetics Uncovers Regulators of Synaptic and Axonal Stability and Degeneration In Vivo, Pathways analysis in kidney cancer using proteomics approach, Pre-treatment plasma proteomic markers associated with survival in oesophageal cancer, A Quantitative Spatial Proteomics Analysis of Proteome Turnover in Human Cells, Reversible molecular pathology of skeletal muscle in spinal muscular atrophy, Evaluation of novel supports for selective and efficient enrichment of phosphorylated peptides, SMN deficiency disrupts brain development in a mouse model of severe spinal muscular atrophy, Cell homeostasis in a Leishmania major mutant overexpressing the spliced leader RNA is maintained by an increased proteolytic activity, Keap1 perceives stress via three sensors for the endogenous signaling molecules nitric oxide, zinc, and alkenals, Detection of oesophageal cancer biomarkers by plasma proteomic profiling of human cell line xenografts in response to chemotherapy, Application of electrospray mass spectrometry to the structural determination of glycosylphosphatidylinositol membrane anchors, A Quantitative Proteomics Analysis of Subcellular Proteome Localization and Changes Induced by DNA Damage, Fate of Glycosylphosphatidylinositol (GPI)-Less Procyclin and Characterization of Sialylated Non-GPI-Anchored Surface Coat Molecules of Procyclic-Form Trypanosoma brucei, W1897 Colorectal Cancer Biomarker Discovery Using Quantitative Proteomics, The Phosphoproteome of Bloodstream Form Trypanosoma brucei, Causative Agent of African Sleeping Sickness, Identification and Specific Localization of Tyrosine-Phosphorylated Proteins in Trypanosoma brucei, In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1, Comparative analysis of the excretory–secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis, Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry, Asparagine Deamidation Perturbs Antigen Presentation on Class II Major Histocompatibility Complex Molecules. Descrição da página. communication in multicellular organisms. Despite the lack of conventional tyrosine kinases in the genome of the single cell An apparently identical disease occurs in multiple species including but not limited to cats, dogs, and rabbits. Low levels of ubiquitously expressed SMN protein result in the degeneration of lower motor neurons, but it remains unclear whether other regions of the nervous system are also affected. Degeneration of synaptic and axonal compartments of neurons is an early event contributing to the pathogenesis of many neurodegenerative diseases, but the underlying molecular mechanisms remain unclear. Douglas Lamont is on Facebook. These authors contributed equally to this work. The second best result is Douglas Lamont age 60s in Heber City, UT. Due to our privacy policy, only current members can send messages to people on ResearchGate. Vê os perfis de pessoas com o nome Douglas Lamont. Por Fábio de Castro, Agência Fapesp Um novo estudo realizado por um grupo de cientistas brasileiros, com colaboração de colegas de outros países, acaba de trazer um novo avanço para a compreensão dos mecanismos de controle da expressão gênica do … that these are not the only surface coat molecules of the procyclic life cycle stage. T. cruzi trypomastigotes (Ty), the classical infective stage, interact with the extracellular matrix (ECM), an obligatory step before invasion of almost all mammalian cells in different tissues. Protein S-glutathionylation is a reversible post-translational modification regulating sulfhydryl homeostasis. Here, we reveal a novel and important role for myelinating glia in regulating synapse elimination at the mo... Low levels of survival of motor neuron (SMN) protein cause the neuromuscular disease spinal muscular atrophy (SMA), characterized by degeneration of lower motor neurons and atrophy of skeletal muscle. Visualize os perfis de pessoas chamadas LaMont Douglas. Author Summary Measuring the properties of endogenous cell proteins, such as expression level, subcellular localization, and turnover rates, on a whole proteome level remains a major challenge in the postgenome era. MSPs. K Murray's 7 research works with 68 citations and 182 reads, including: Supplementary Data Tables 5–9 Nuclear actin and myosin are essential regulators of gene expression. Join Facebook to connect with Douglas Lamont and others you may know.
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